Autoradiographic method for studying absorption and translocation of herbicides using C14-labeled compounds
A. S. Crafts
Authors AffiliationsS. Yamaguchi was Senior Laboratory Technician in the Department of Botany, Davis; A. S. Crafts was Professor of Botany and Botanist in the Experiment Station, Davis.
Hilgardia 28(6):161-191. DOI:10.3733/hilg.v28n06p161. December 1958.
Various techniques and methods evolved for studying absorption and translocation of herbicides using corbon14-labeled compounds are presented. Advantages of the counting method and of the autoradiographic method are compared, and aspects of plant physiology related to absorption and translocation are discussed. The importance of the autoradiographic method for routine study is emphasized, and complete details of this method are described: choice of plants; greenhouse culture of plants; the specific activity of the tracer; the stock solutions; the treatment solutions; application of the solutions; the number of plants needed for an. experiment; treatment time; freeze-killing and freeze-drying; oven drying; open-air drying; autoradiographing; filing of the autoradiographs; and copying of the autoradiographs.
Research and method development reported in this paper was made possible by A. E. C. Contract AT(11-1)-34 Project No. 9.
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