University of California

Factors affecting the aseptic culture of Lovell peach seedlings


Dale E. Kester

Author Affiliations

Dale E. Kester was Lecturer in Pomology and Assistant Pomologist in the Experiment Station, Davis.

Publication Information

Hilgardia 22(9):335-365. DOI:10.3733/hilg.v22n09p335. September 1953.

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THIS PAPER PRESENTS investigation of factors affecting the germination and growth of Lovell peach embryos. Presence of the cotyledons, length of after-ripening, sucrose, light, and a number of accessory substances are considered. The technique used consisted of aseptic stratification of Lovell peach seeds followed by the aseptic culture of the embryos on nutrient agar.

The studies showed that reduction in the amount of cotyledons reduced over-all growth, and their complete removal prevented germination. With limiting amounts of the cotyledons the additional specific effect of inhibiting internode elongation appeared. Root length was not reduced until about 90 per cent of the cotyledons were removed. Injury resulted in initial stimulation to shoot growth.

Addition of sucrose to the medium tended to be inhibiting when large amounts of cotyledons were present but became stimulating as they were reduced, and with ecotyledonized embryos allowed germination, though growth was maintained for only 10 to 14 days, a rosettelike shoot developed, and root tips became blackened. Increasing the length of after-ripening tended to increase growth. In darkness, internode inhibition was not present, but inhibition in root elongation was evident and the cotyledons did not overcome root-tip blackening.

The function of the cotyledons seemed to be twofold: (1) to produce growth of shoots, leaves, and roots; and (2) to provide a stimulus for growth after germination had started. As little as 1/16 of the cotyledons could carry out both functions. The effect of the cotyledons could not be replaced by any of the accessory substances tested, though some of them had a slight stimulating effect on shoot or root growth or both.

A similarity between growth from ecotyledonized embryos (embryos with cotyledons removed), growth of excised immature embryos, and growth from non-after-ripened embryos is pointed out and the significance discussed.

Literature Cited

Addicott F. T. Vitamin B1 in relation to meristematic activity of isolated pea roots. Bot. Gaz. 1939. 100:836-43.

Avery G. S. Jr., Berger J., Shalucha B. Total auxin extraction from wheat. Amer. Jour. Bot. 1942. 29:612-16. DOI: 10.2307/2437172 [CrossRef]

Baker G. A., Davis L. D. Growth of the cheek diameter of peaches. Amer. Soc. Hort. Sci. Proc. 1951. 57:104-10.

Berger J., Avery G. S. Jr. Isolation of an auxin precursor and an auxin (indoleacetic acid) from maize. Amer. Jour. Bot. 1944. 31:199-203. DOI: 10.2307/2437605 [CrossRef]

Blakeslee A. F., Satina S. New hybrids from incompatible crosses in Datura through culture of excised embryos on malt media. Science. 1944. 99:331-34. DOI: 10.1126/science.92.2400.589 [CrossRef]

Blociszewsky T. Physiologische Untersuchungen über die Keimung und weitere Entwicklung einzemner samentiele bedecktsamiger Pflanzen. Landw. Jahrb. von Nathusius v. Thiel. 1876. 5:145 (Quoted by Brown and Morris, 1890.)

Bonner J. Nicotinic acid and the growth of isolated pea embryos. Plant Physiol. 1938. 13:865-68. DOI: 10.1104/pp.13.4.865 [CrossRef]

Bonner J., Axtman G. The growth of plant embryos in vitro. Preliminary experiments on the role of accessary substances. Natl. Acad. Sci. Proc. 1937. 23:453-57.

Bonner J., Bonner H. The B vitamins as plant hormones. Vitamins and Hormones. 1948. 6:225-75. DOI: 10.1016/S0083-6729(08)60459-8 [CrossRef]

Brink R. A., Cooper D. C., Ausherman L. E. Hybrid between Hordeum jubatum and Secale cereale reared from an artificially cultivated embryo. Jour. Hered. 1944. 35:67-75.

Brown H. T., Morris G. H. Researches on the germination of some of the Gramineae. Jour. Chem. Soc. London. 1890. 57:458-528.

Cooper W. C. Hormones and root formation. Bot. Gaz. 1938. 99:599-614. DOI: 10.1086/334734 [CrossRef]

Davidson O. W. The germination of “non-viable” peach seeds. Amer. Soc. Hort. Sci. Proc. 1933. 30:129-33.

Dietrich K. Über die Kulture von Embryonen ausserhalf der Samen. Flora N. F. 1924. 17:379-417.

Flemion F., Waterbury E. Further studies with dwarf seedlings of non-after-ripened peach seeds. Boyce Thompson Inst. Contrib. 1945. 13:415-22.

Gilmore A. E. A technique for embryo culture of peaches. Hilgardia. 1950. 20:147-69.

Haberlandt G. Die Schutzeinrichtungen in der Entwicklung der Keimpflanze. 1877. Eine biologische Studie, Wien. (Quoted by Stingl, 1907) DOI: 10.5962/bhl.title.50571 [CrossRef]

Hall C. B. Culture of Solanum nigrum embryos. Amer. Soc. Hort. Sci. Proc. 1948. 52:343-46.

Hannig E. Über die Cultur von Cruciferen-Embryonen. Bot. Zeitung. 1904. 62:45-80.

Hoagland D. R., Arnon D. I. The water-culture method for growing plants without soil. California Agr. Exp. Sta. Cir. 1938. 347:1-39.

Jorgensen C. A. The experimental formation of heteroploid plants in the genus Solarium. Jour. Genet. 1928. 19:133-211. DOI: 10.1002/gepi.1370170732 [CrossRef]

Kent N., Brink R. A. Growth in vitro of immature Hordeum embryos. Science. 1947. 106:547-48. DOI: 10.1522/25023117 [CrossRef]

Kögl Fritz, Haagen-Smit A. J. Biotin und Aneurin als Phytohormone. Hoppe-Seylers Ztschr. f. Physiol. Chem. 1936. 243:215-26.

Laibach F. Ectogenesis in plants. Jour. Hered. 1929. 20:201-08.

Lammerts W. E. Embryo culture an effective technique for shortening the breeding cycle of deciduous trees and increasing germination of hybrid seed. Amer. Jour. Bot. 1942. 29:166-71. DOI: 10.2307/2437446 [CrossRef]

LaRue C. D. The growth of plant embryos in culture. Torrey Bot. Club Bul. 1936. 63:365-82. DOI: 10.2307/2480944 [CrossRef]

Lee F. A., Tukey H. B. Chemical changes accompanying growth and development of seed and fruit of the Elberta peach. Bot. Gaz. 1942. 104:348-55. DOI: 10.1086/335139 [CrossRef]

Lofland H. B. Jr. In vitro culture of the cotton embryo. Bot. Gaz. 1950. 111:307-11.

Loo S. W., Wang F. H. The culture of young conifer embryos in vitro. Science. 1943. 98:544

Merry J. Studies on the embryo of Hordeum sativum II. The growth of the embryo in culture. Torrey Bot. Club Bul. 1942. 69:360-72.

Nickell L. B. Embryo culture of weeping crabapples. Amer. Soc. Hort. Sci. Proc. 1951. 57:401-05.

Prat S. The toxicity of tissue juices for cells of the tissue. Amer. Jour. Bot. 1927. 14:120-25. DOI: 10.2307/2435602 [CrossRef]

Rytz W. Jr. Beitrag zum Aneurinstoffwechsel bei hoheren Pflanzen. Schweitz. Bot. Gesell. Ber. 1939. 48:339-99.

Sachs J. Zur Keimunggeschichte der Gräser. Bot. Ztg. 1862. 20:148 (Quoted by Stingl, 1907)

Sanders M. F., Burkholder P. R. Influence of amino acids on growth of Datura embryos in culture. Natl. Acad. Sci. Proc. 1948. 34:516-26.

Smith P. G. Embryo culture of a tomato species hybrid. Amer. Soc. Hort. Sci. Proc. 1944. 44:413-16.

Snedecor G. W. Statistical methods. 1946. 4th ed. Ames, Iowa: Iowa State College Press. 485p. DOI: 10.1097/00010694-195702000-00023 [CrossRef]

Stewart W. S. A plant growth inhibitor and plant growth inhibition. Bot. Gaz. 1939. 101:91-108. DOI: 10.1086/334853 [CrossRef]

Stingl G. Experimentelle Studie über die Ernährung von pflanzlichen Embryonen. Flora. 1907. 97:308-31.

Street H. E., Lowe J. S. The carbohydrate nutrition of tomato roots. II. The mechanism of sucrose absorption by excised roots. Ann. Bot. [London] n. s. 1950. 14:307-29.

Tukey H. B. Artificial culture of sweet cherry embryo. Jour. Hered. 1933a. 24:7-12.

Growth of the peach embryo in relation to growth of fruit and season of ripening. Amer. Soc. Hort. Sci. Proc. 1933b. 30:209-18.

Artificial culture methods for isolated embryos of deciduous fruits. Amer. Soc. Hort. Sci. Proc. 1934. 32:313-22.

Embryo abortion in the peach in relation to chemical composition and season of fruit ripening. Bot. Gaz. 1937. 98:586-97. DOI: 10.1086/334661 [CrossRef]

Growth patterns of plant development from immature embryos in artificial culture. Bot. Gaz. 1938. 99:630-65. DOI: 10.1086/334736 [CrossRef]

van Overbeek J., Conklin M. E., Blakeslee A. F. Cultivation in vitro of small Datura embryos. Amer. Jour. Bot. 1942. 29:472-77.

van Tieghem P. Recherches physiologiques sur la germination. Ann. Sci. Nat. Bot. 1873. 17:205-24.

von Hausen S. S. On the role of growth substances in higher plants. Physiol. Plantarum. 1948. 1:85-94. DOI: 10.1111/j.1399-3054.1948.tb07112.x [CrossRef]

Went F. W. Specific factors other than auxin affecting growth and root formation. Plant Physiol. 1938. 13:55-80. DOI: 10.1104/pp.13.1.55 [CrossRef]

Kester D. 1953. Factors affecting the aseptic culture of Lovell peach seedlings. Hilgardia 22(9):335-365. DOI:10.3733/hilg.v22n09p335
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