In vitro gas production provides effective method for assessing ruminant feeds

DePeters, E; Robinson, P; Getachew, G

In vitro gas production provides effective method for assessing ruminant feeds

Authors

Edward J. DePeters
Peter H. Robinson
Girma Getachew

Authors Affiliations

E.J. DePeters is Professor, Department of Animal Science, UC Davis. This research was supported by the California Agricultural Experiment Station, UC Davis; P.H. Robinson is Cooperative Extension Specialist, Department of Animal Science, UC Davis. This research was supported by the California Agricultural Experiment Station, UC Davis; G. Getachew is Postdoctoral Associate, Department of Animal Science, UC Davis. This research was supported by the California Agricultural Experiment Station, UC Davis.

Publication Information

Hilgardia 58(1):54-58. DOI:10.3733/ca.v058n01p54. January 2004.

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Abstract

An animal's feed intake, and how well that feed is digested, determine the feed's production performance. The in vitro gas production technique is a relatively simple method for evaluating feeds, as large numbers of samples can be incubated and analyzed at the same time. This method has been applied successfully at UC Davis for a variety of purposes in feed evaluation, including calculating organic matter digestibility, the metabolizable energy of feeds and kinetics of their fermentation; determining how feed value is affected by added fat, antinutritive factors and rumen modifiers; quantifying the energy value of feed mixtures (rations); monitoring microbial change in the rumen; synchronizing nutrient digestion; and selecting forage nutrient targets for agricultural biotechnology. More than half of the nutrients consumed by ruminant animals leave the animal unutilized and undigested, and are excreted in feces, urine and gases. The in vitro gas production method can be used to examine animal waste components that impact the environment and develop appropriate mitigations.

References

DePeters EJ, Getachew G, Fadel JG, et al. In vitro rumen gas production as a method to compare fermentation characteristics of steam-flaked corn. Animal Feed Sci Technol. 2003. 105:109-22. https://doi.org/10.1016/S0377-8401(03)00042-7

Getachew G, Crovetto GM, Fondevila M, et al. Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy value of ruminant feeds. Animal Feed Sci Technol. 2002. 102:169-80. https://doi.org/10.1016/S0377-8401(02)00212-2

Getachew G, DePeters EJ, Robinson PH, Taylor SJ. In vitro rumen fermentation and gas production: Influence of yellow grease, tallow, corn oil and their potassium soaps. Animal Feed Sci Technol. 2001. 93:1-15. https://doi.org/10.1016/S0377-8401(01)00264-4

Getachew G, Makkar HPS, Becker K. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. Br J Nutr. 2000a. 84:73-83. PubMed PMID: 10961163

Getachew G, Makkar HPS, Becker K. Tannins in tropical browses: Effects on in vitro microbial fermentation and microbial protein synthesis in media containing different amounts of nitrogen. J Agric Food Chem. 2000b. 48:3581-8. https://doi.org/10.1021/jf990740v PubMed PMID: 10956154

Johnson KA, Johnson DE. Methane emissions from cattle. J Animal Sci. 1995. 73:2483-92.

Kuelling DR, Menzi H, Sutter F, et al. Ammonia, nitrous oxide and methane emissions from differently stored dairy manure derived from grass- and hay-based rations. Nutrient Cycling Agroecosystems. 2003. 65:13-22. https://doi.org/10.1023/A:1021857122265

Liu JX, Susenbeth A, Sudekum KH. In vitro gas production measurements to evaluate interactions between untreated and chemically treated rice straw, grass and mulberry leaves. J Animal Sci. 2002. 80:517-24.

Makkar HPS, Blümmel M, Becker K. In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. J Sci Food Agric. 1995. 69:481-93. https://doi.org/10.1002/jsfa.2740690413

Muetzel S, Becker K. Supplementation of barley straw with Sesbania pachycarpa leaves in vitro: Effects of fermentation variables and rumen microbial population structure quantified by ribosomal RNA-targeted probes. Br J Nutr. 2003. 89:445-53. https://doi.org/10.1079/BJN2002813 PubMed PMID: 12654162

Siaw DEK, Osuji PO, Nsahlai IV. Evaluation of multipurpose tree germplasm: The use of gas production and rumen degradation characteristics. J Agric Sci (Cambridge). 1993. 120:319-30.

Tilley JM, Terry RA. A two-stage technique for the in vitro digestion of forage crops. J Br Grassland Soc. 1963. 18:104-11.

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