University of California

The effect of riboflavin and the filtrate factor on egg production and hatchability


S. Lepkovsky
L. W. Taylor
T. H. Jukes
H. J. Almquist

Authors Affiliations

S. Lepkovsky was Associate Professor of Poultry Husbandry and Associate Poultry Husbandman in the Experiment Station; L. W. Taylor was Associate Professor of Poultry Husbandry and Associate Poultry Husbandman in the Experiment Station; T. H. Jukes was Instructor in Poultry Husbandry and Junior Poultry Husbandman in the Experiment Station; H. J. Almquist was Assistant Professor of Poultry Husbandry and Assistant Poultry Husbandman in the Experiment Station.

Publication Information

Hilgardia 11(10):559-591. DOI:10.3733/hilg.v11n10p559. September 1938.

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Numerous investigations(Bethke and Kennard, 1928); (McFarlane, Fulmer, and Jukes, 1930); (Titus, Byerly, and Ellis, 1933)6 have indicated a factor or factors required for hatchability other than the fatsoluble vitamins. The factor or factors seemed associated with some protein concentrates and with such feedstuffs as alfalfa, milk, liver, yeast, and fresh greens. Later work (Halpin, Holmes, and Hart, 1933); (Bethke, Record, and Kennard, 1933); (Heiman, 1935) indicated that vitamin G was the factor responsible. It is now known (Birch, György, and Harris, 1935) that the term “vitamin G” as previously used in the literature covered the complex action of several vitamins. (Bethke, Record, and Kennard (1936)) have suggested that riboflavin was the active factor responsible for increased hatchability. On the other hand, (Smith and Branion (1936)) have expressed the opinion that more than one factor in the vitamin-G complex7 is involved in hatchability of eggs.

Literature Cited

Almquist H. J., Stokstad E. L. R. Hemorrhagic chick disease of dietary origin. Jour. Biol. Chem. 1935. 111:105-13.

Bender R. C., Supplee G. C. Significance of quantitative relationships in vitamin B complex studies. Jour. Amer. Chem. Soc. 1937. 59:1178-82.

Bethke R. M., Kennard D. C. Producing hatchable eggs. Ohio Agr. Exp. Sta. Bimonthly Bul. 1928. 13:210-16. DOI: 10.3382/ps.0250579 [CrossRef]

Bethke R. M., Record P. R., Kennard D. C. Relation of the vitamin G complex to hatchability and the nutritive value of eggs. Poultry Sci. 1933. 12:332-33.

Bethke R. M., Record P. R., Kennard D. C. The relation of vitamin G to the hatchability of hens’ eggs. Jour. Nutrition. 1936. 12:297-307.

Birch T. W., György P., Harris L. J. The vitamin B2 complex. Differentiation of the antiblack tongue and the “P-P” factors from lactoflavin and vitamin B6 (so-called “rat pellagra” factor). Biochem. Jour. 1935. 19:2830-50.

Bourquin A., Sherman H. C. Quantitative determination of vitamin G (B2). Jour. Amer. Chem. Soc. 1931. 53:3501-5.

Davis H. J., Norris L. C., Heuser G. F.

Davis H. J., Norris L. C., Heuser G. F. The role of vitamin G in reproduction in poultry. Poultry Sci. 1938a. 17:81-86. DOI: 10.3382/ps.0170081 [CrossRef]

Davis H. J., Norris L. C., Heuser G. F. Further evidence on the amount of vitamin G required for reproduction in poultry. Poultry Sci. 1938b. 17:87-93. DOI: 10.3382/ps.0170087 [CrossRef]

Elvehjem C. A., Koehn C. J. Jr. Studies on vitamin B2 (G). The non-identity of vitamin B2 and the flavins. Jour. Biol. Chem. 1935. 108:709-28.

Euler H. V., Karrer P., Malmberg M., Schopp K., Benz F., Becker B., Frei P. Synthese des Lactoflavins (vitamin B2) und anderer Flavine. Helvetica Chim. Acta. 1935. 18:522-35.

Halpin J. G., Holmes C. E., Hart E. B. Vitamin G inadequate in many rations for laying hens. Wisconsin Agr. Exp. Sta. Bul. 1933. 425:18-19. In: Christensen, C. L., and Noble Clark. Facts for farmers.

Heiman V. The relative vitamin G content of dried whey and dried skim milk. Poultry Sci. 1935. 14:137-46.

Jukes T. H. Biological assay of lactoflavin with chicks. Jour. Nutrition. 1937a. 14:223-33.

Jukes T. H. Further observations on the assay, distribution, and properties of the filtrate factor. Jour. Biol. Chem. 1937b. 117:11-20.

Kohls Clara L. Blood picture in vitamin-G deficiency. [Abstract.] Anat. Rec. 1932. 52:62

Kuhn R., György P., Wagner-Jauregg T. Über Lactoflavin, den Farbstoff der Molke. Ber. Deut. Chem. Gesell. 1933. 66:1934-38.

Kuhn R., Rudy H., Wagner-Jauregg T. Über Lactoflavin (vitamin B2). Ber. Deut. Chem. Gesell. 1933. 66:1950-56.

Lepkovsky S., Jukes T. H. The vitamin G requirements of the chick. Jour. Biol. Chem. 1935. 111:119-31.

Lepkovsky S., Jukes T. H., Krause M. E. The multiple nature of the third factor of the vitamin B complex. Jour. Biol. Chem. 1936. 115:557-66.

Lyons M., Insko W. M. Jr. Chondrodystrophy in the chick embryo produced by manganese deficiency in the diet of the hen. Kentucky Agr. Exp. Sta. Bul. 1937. 371:63-75.

McCollum E. V., Simmonds N. A study of the dietary essential water-soluble B in relation to the solubility and stability towards reagents. Jour. Biol. Chem. 1918. 33:55-89.

McFarlane W. D., Fulmer H. L., Jukes T. H. Studies in embryonic mortality in chicks. I. The effect of diet upon the nitrogen, tyrosine, tryptophane, cystine, and iron content of the proteins and on the total copper of the hen’s eggs. Biochem. Jour. 1930. 24:1611-30.

Poley W. E., Moxon A. L., Franke K. W. Further studies of the effects of selenium poisoning on hatchability. Poultry Sci. 1937. 16:219-25. DOI: 10.3382/ps.0160219 [CrossRef]

Ringrose A. T., Norris L. C., Heuser G. F. The occurrence of a pellagra-like syndrome in chicks. Poultry Sci. 1931. 10:166-77. DOI: 10.3382/ps.0100166 [CrossRef]

Sanford A. H., Sheard C., Osterberg A. E. The photelometer and its use in the clinical laboratory. Amer. Jour. Clin. Path. 1933. 3-405-20.

Sebrell W. H., Onstott R. H., Hunt D. J. The treatment of black tongue with a preparation containing the “filtrate factor” and evidence of riboflavin deficiency in dogs. Pub. Health Repts. [U. S.]. 1937. 52:427-33.

Sherman H. C., Smith S. L. The vitamins. 1931. 2nd ed. New York, N. Y.: The Chem. Catalog. Co. Inc. DOI: 10.1001/archsurg.1963.01310080011003 [CrossRef]

Smith J. B., Branion H. D. Hatchability studies with certain feeds. Wiss. Ber. des VI. Weltgeflügel Kong. 1936. 2: Germany: Uschmann in Weimar. p. 77-83.

Titus H. W., Byerly T. C., Ellis N. R. Effect of diet on egg composition. I. Partial chemical analysis of eggs produced by pullets on different diets. Jour. Nutrition. 1933. 6:127-38.

Lepkovsky S, Taylor L, Jukes T, Almquist H. 1938. The effect of riboflavin and the filtrate factor on egg production and hatchability. Hilgardia 11(10):559-591. DOI:10.3733/hilg.v11n10p559
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