University of California

The adaptation of qualitative and quantitative techniques for determination of radioactive dalapon in plant tissues


Chester L. Foy

Author Affiliations

Chester L. Foy was Lecturer in Botany, and Assistant Botanist in the Experiment Station, Davis.

Publication Information

Hilgardia 30(5):153-173. DOI:10.3733/hilg.v30n05p153. July 1960.

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In studying the absorption, distribution, and metabolism of dalapon (2,2-dichloropropionic acid) in relation to phytotoxicity, a number of simplified techniques were developed, and other established procedures were usefully modified. The techniques of autoradiography, extraction and fractionation, counting, and paper partition cochromatography were effectively combined, thus permitting qualitative and quantitative determination of distribution patterns and, to some extent, of the metabolic fate of translocated herbicides. Although this report is concerned specifically with dalapon, the approach employed with that compound should be applicable, with minor changes, to the study of others.

Physiological findings resulting from application of these techniques will be published separately.

Literature Cited

Arnon D. I., Stout P. R., Sipos F. Radioactive phosphorus as an indicator of phosphorus absorption of tomato fruits at various stages of development. Amer. Jour. Bot. 1940. 27:791-98. DOI: 10.2307/2436908 [CrossRef]

Aronoff Sam. Techniques of radiobiochemistry. 1956. Ames, Iowa: Iowa State College Press. 228p.

Aronoff S., Benson A., Hazzid W. Z., Calvin M. Distribution of C14 in photosynthesizing barley seedlings. Science. 1947. 105:664-65. DOI: 10.1126/science.105.2739.664 [CrossRef]

Benson A. A., Bassham J. A., Calvin M., Goodale T. E., Haas V. A., Stepka W. The path of carbon in photosynthesis. V. Paper chromatography and radio-autography of the products. Jour. Amer. Chem. Soc. 1950. 72:1710-18. DOI: 10.1021/ja01160a080 [CrossRef]

Blanchard F. A. Uptake, distribution and metabolism of carbon-14-labeled trichloroacetate in corn and pea plants. Weeds. 1954. 3(3):274-78. DOI: 10.2307/4039978 [CrossRef]

Boyd G. A. Autoradiography in biology and medicine. 1955. New York: Academic Press. 399p.

Calvin M. C., Heidelburger C., Reid J. C., Tolbert B. M., Yankwich P. E. Isotopic carbon. 1949. New York: John Wiley and Sons, Inc. 376p.

Colwell R. N. The use of radioactive phosphorus in translocation studies. Amer. Jour. Bot. 1942. 29:798-807. DOI: 10.2307/2437645 [CrossRef]

Comar C. L. Radioisotopes in biology and agriculture. 1955. New York: McGraw-Hill Book Company, Inc. 481p.

Consden R. A., Gordon A. H., Martin A. J. P. Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. Jour. 1944. 38:225-32.

Crafts A. S. Herbicides—their absorption and translocation. Agr. and Food. Chem. 1953. 1:51-55. DOI: 10.1021/jf60001a006 [CrossRef]

Crafts A. S. Translocation of herbicides. I. The mechanism of translocation: methods of study with C14-labeled 2,4-D. Hilgardia. 1956. 26(6):287-334. DOI: 10.3733/hilg.v26n06p287 [CrossRef]

Manganelli R. M., Brofazi F. R. Quantitative determination of volatile fatty acids by paper chromatography for application to sewage sludge digestion. Anal. Chem. 1957. 29(10):1441-43. DOI: 10.1021/ac60130a012 [CrossRef]

Meikle R. W. Personal communication 1956.

Meikle R. W. Personal communication 1957.

Nelson C. D., Gorham P. R. Translocation of radioactive sugars in the stems of soybean seedlings. Can. Jour. Bot. 1957. 35:703-13. DOI: 10.1139/b57-060 [CrossRef]

Pallas J. E. Jr., Crafts A. S. Critical preparation of plant material for autoradiography. Science. 1957. 125:192-93. DOI: 10.1126/science.125.3240.192 [CrossRef]

Redemann E. T., Meikle R. W. Isotope dilution technique for the determination of pesticide residues. Adv. in Pest Control Research. 1958. 2:183-206.

Renard M. Paper chromatography of chlorinated acids. A new, simple and rapid method to establish the structure of dipeptides. Bull. des Soc. Chem. Belges. 1949. 58:301-09. DOI: 10.1002/bscb.19490580703 [CrossRef]

Roberts H. R., Bucek W. Rapid procedure for separating C2 and C6 volatile fatty acids by horizontal paper chromatography at elevated temperatures. Anal. Chem. 1957. 29(10):1447-49. DOI: 10.1021/ac60130a014 [CrossRef]

Smith Grant N. Counting of radioactive dalapon in biological systems. Down to Earth. 1958. 14(2):3-6. 12.

Smith G. N., Getzendaner M. E., Kutchinski A. H. Determination of 2,2-dichloropropionic acid (dalapon) in sugar cane. Agr. and Food Chem. 1957. 5(9):675-78. DOI: 10.1021/jf60079a003 [CrossRef]

van Etten Cecil, Mcgrew Clara E. Ion exchange micromethods for separation of fermentation acids. Anal. Chem. 1957. 29:1506-09. DOI: 10.1021/ac60130a036 [CrossRef]

Yamaguchi S., Crafts A. S. Autoradiographic method for studying absorption and translocation of herbicides using C14-labeled compounds. Hilgardia. 1958. 28(6):161-91. DOI: 10.3733/hilg.v28n06p161 [CrossRef]

Foy C. 1960. The adaptation of qualitative and quantitative techniques for determination of radioactive dalapon in plant tissues. Hilgardia 30(5):153-173. DOI:10.3733/hilg.v30n05p153
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