A technique for embryo culture of peaches
AuthorA. E. Gilmore
Author AffiliationsA. E. Gilmore was Assistant Specialist in the Experiment Station.
Hilgardia 20(8):147-170. DOI:10.3733/hilg.v20n08p147. September 1950.
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This publication describes a technique. …
for growing peach-embryo cultures. In laboratory experiments, a high percentage of the cultures was free of microorganisms that would develop under yeast-extract broth within 15 days at room temperature.
The salient feature of the technique is a “sterilization” procedure on dry, dormant peach seeds, followed by maintenance of aseptic conditions during the subsequent after-ripening. Merthiolate 1:2,000 in 50 per cent alcohol was found to be suitable for the purpose.
Various factors influencing the growth of peach-embryo cultures are discussed. Suggestions are also made for establishing a standard practice in growing peach-embryo cultures to be used for biological testing.
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