Meiosis in Allium fistulosum, Allium cepa, and their hybrid
AuthorsS. L. Emsweller
H. A. Jones
Authors AffiliationsS. L. Emsweller was Assistant Professor of Truck Crops and Assistant Olericulturist in the Experiment Station; H. A. Jones was Professor of Truck Crops and Olericulturist in the Experiment Station.
Hilgardia 9(5):275-294. DOI:10.3733/hilg.v09n05p275. March 1935.
Abstract does not appear. First page follows.
A Preceding paper(4) discussed the general morphology of several varieties of Allium. cepa L., Allium fistulosum L., and their hybrids.
Because of the high degree of self-sterility in the hybrid, cytological studies were begun in 1932 in the hope that they might explain the cause of sterility and possibly indicate whether a stable derivative combining the desirable characters of the two species might be secured. These investigations have now been completed on the Yellow Globe Danvers variety of Allium cepa (pedigree 15-108-1), on a strain of fistulosum (37-1), and on their hybrid.
To secure material containing pollen mother cells at desirable stages, an anther was first crushed in aceto carmine and examined under low power. If suitable division figures were found, the remaining five anthers of a flower were removed and fixed, for at least 24 hours, in a solution of 25 per cent acetic acid and 75 per cent absolute alcohol. They were then transferred to 80 per cent alcohol, where they may be kept indefinitely. Material stored for four months and then stained has given better differentiation between chromatin and cytoplasm than material used a few days after fixing.
When preparations were made from fixed material, an anther was placed on a slide and covered with a small drop of aceto carmine. The aceto carmine had to contain a considerable amount of iron; otherwise the chromosomes were stained very weakly, and differentiation was not sharp. A curved, blunt needle was used to press out the pollen mother cells, and the anther wall and all other débris were carefully removed before the cover slip was added. All excess aceto carmine was wiped off, the slide examined under low power, and its preparation completed under observation. Usually the cells were found in masses scattered over the slide.
 Avery P. Cytological studies of five interspecific hybrids of Crepis leontodontoides. Univ. California Pubs. Agr. Sci. 1930. 6:135-167.
 Belling J. Crossing over and gene rearrangement in flowering plants. Genetics. 1933. 18:388-413.
 Darlington C. D. Chromosome behavior and structural hybridity in the Tradescantiae. Jour. Genetics. 1929. 21:207-286.
 Emsweller S. L., Jones H. A. An interspecific cross in Allium. Hilgardia. 1935. 9:265-273. DOI: 10.3733/hilg.v09n05p265 [CrossRef]
 Hollingshead L. Cytological investigations of hybrids and hybrid derivatives of Crepis capillaris and Crepis tectorum. Univ. California Pubs. Agr. Sci. 1930. 6:55-94.
 Ishikawa C. Die Entwicklung der Pollenkörner von Allium fistulosum L ein Beitrag zur Chromosomen reduction im Pflanzenreich. Tokyo Imp. Univ. Col. Sci. Jour. 1897. 10:193-224.
 Levan A. Cytological studies in Allium IV. Allium fistulosum. Svensk Bot. Tidskr. 1933. 27:211-232.
 Lündegardh H. Chromosomen, Nukleolen und die Veränderungen im Protoplasma bei der Karyokinese. Beitr. Biol. Pflanz. 1912. 11:373-424.
 McClintock B. A method for making aceto-carmin smears permanent. Stain Technol. 1929. 4:53-56.
 Miyake K. Ueber Reductionsteilung in den Pollenmutterzellen einiger Monokotylen. Jahrb. Wiss. Bot. 1905. 42:83-120.
 Nemec B. Ueber die karyokinetische Kerntheilung in der Wurzelspitze von Allium cepa. Jahrb. Wiss. Bot. 1899. 33:313-336.
 Sax K. The cytological mechanism of crossing over. Jour. Arnold Arboretum. 1932. 13:180-212.
 Schaffner J. H. Karyokinesis in the root tips of Allium cepa. Bot Gaz. 1898. 26:225-238.
 Strasburger E. Ueber Reductionsteilung, Spindelbildung, Centrosomen und Cilienbildner im Pflanzenreich. Hist. Beitr. 1900. 6:1-224.
 Taylor W. R. The chromosome morphology of Velthemia, Allium and Cyrtanthus. Amer. Jour. Bot. 1925. 12:104-115.
 Weinrich D. H. The spermatogenesis of Phrynotettix. Bul. Mus. Compar. Zoöl. [Harvard]. 1916. 60:57-135.
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An interspecific hybrid in Allium