University of California

Electrophoresis of tobacco mosaic virus


William N. Takahashi
T. E. Rawlins

Authors Affiliations

William N. Takahashi was Graduate student in Plant Pathology; T. E. Rawlins was Assistant Professor of Plant Pathology and Assistant Plant Pathologist in the Experiment Station.

Publication Information

Hilgardia 4(15):441-463. DOI:10.3733/hilg.v04n15p441. April 1930.

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Iwanowski’s(34) demonstration in 1892 that the mosaic disease of tobacco is caused by a filter-passing agent opened a new and extensive field of research in the pathology of both plants and animals. Since then a number of different virus4 diseases have been found affecting a variety of plants and animals. The bacteriophage has also been considered a virus by many workers.

Much literature dealing with various phases of virus diseases has been published. In the following review we have attempted to abstract only the work giving evidence as to the nature of the viruses.

Review of Literature

Cytological Studies.—For some time: pathologists resorted to cytological methods in their search for a visible parasite causing the virus diseases. A great deal of careful work has been done in this field. Iwanowski,(35) Dickson,(21) McKinney, Eckerson and Webb,(45) Goldstein,(30) Palm,(54) Rawlins and Johnson,(58) Hoggan,(32) Kunkel,(38) Holmes,(33) Smith, (61) and various students of animal pathology have described abnormal intracellular bodies in virus-infected host tissues. The structure of these bodies is not sufficiently distinct to warrant the conclusion that they are a stage of a causal organism. In fact, many of the workers are inclined to the view that they are products of the causal agent or of the diseased host cell.

Literature Cited

[1] Allard H. A. The mosaic diseases of tobacco. U. S. Dept. Agr. Bul. 1914. 40:1-34. DOI: 10.1126/science.36.938.875 [CrossRef]

[2] Allard H. A. Effect of dilution on the infectivity of the mosaic disease of tobacco. Jour. Agr. Res. 1915. 3:295-299.

[3] Allard H. A. Some properties of the virus of the mosaic disease of tobacco. Jour. Agr. Res. 1916. 6:649-672.

[4] Arnold L., Weiss E. Isolation of bacteriophage free from bacterial proteins. Jour. Infect. Dis. 1925. 37:411-417. DOI: 10.1093/infdis/37.5.411 [CrossRef]

[5] Arthur J. M., Newell J. M. The killing of plant tissue and the inactivation of tobacco mosaic virus by ultra-violet radiation. Amer. Jour. Bot. 1929. 16:338-353. DOI: 10.2307/2435784 [CrossRef]

[6] Baker S. L., Nanavutty S. H. A quantitative study of the effect of ultra-violet rays upon bacteriophage. Brit. Jour. Exp. Path. 1929. 10:45-60.

[7] Bayliss W. M. Principles of general physiology. 1924. Fourth ed. London: Longmans, Green, and Co. DOI: 10.1097/00005053-192203000-00083 [CrossRef]

[8] Beijeirinck M. W. Ueber ein Contagium vivum fluidum als Ursache der Fleckenkrankheit der Tabaksblaetter. Centbl. Bakt. 1899. 5:27-33.

[9] Bertrand G. Etude biochimique de la bacterie du sorbose. Ann. Chim. et Phys. 1904. 3:181-288.

[10] Bertrand G., Thomas P. Practical biological chemistry. 1920. London: G. Bell and Sons, Ltd. 348p.

[11] Bovie W. T. The action of light on protoplasm. Amer. Jour. Trop. Dis. and Prev. Med. 1915. 2:506-517.

[12] Brewer P. H., Kraybill H. R., Gardner M. W. Purification of the virus of tomato mosaic. Phytopath. 1927. 17:744

[13] Bronfenbrenner J. Does bacteriophage respire?. Science. 1926. 63: p. 51-52. DOI: 10.1126/science.63.1619.51 [CrossRef]

[14] Bronfenbrenner J. Further evidence of the resistance of bacteriophage to alcohol. Proc. Soc. Exper. Biol. 1926-27. 24:373 DOI: 10.3181/00379727-24-3378 [CrossRef]

[15] Carsner E. Attenuation of virus of sugar beet curly-top. Phytopath. 1925. 15:745-758.

[16] Chapman G. H. Mosaic disease of tobacco. Massachusetts Agr. Exp. Sta. Bul. 1917. 175:73-117. DOI: 10.1126/science.43.1111.537 [CrossRef]

[17] Clark W. M. The determination of hydrogen ions. 1920. Baltimore: Williams and Wilkins Company. 317p. DOI: 10.1097/00000441-192901000-00018 [CrossRef]

[18] Cleveland L. R. Some problems which may be studied by oxygenation. Science. 1926. 63:168-170. DOI: 10.1126/science.63.1623.168 [CrossRef]

[19] Cohn E. J., Gross J., Johnson O. C. Isoelectric point of the proteins in certain vegetable juices. Jour. Gen. Physiol. 1919-20. 2:145-160. DOI: 10.1085/jgp.2.2.145 [CrossRef]

[20] De Groat A. F. The bacteriophage: A method of isolation. Jour. Immunol. 1927. 14:175-179.

[21] Dickson B. T. Studies concerning mosaic diseases. McDonald College Tech. Bul. 1922. 2:1-125.

[22] Douglas S. R., Smith Wilson. Cataphoresis experiment with the virus of vaccinia, Brit. Jour. Exp. Path. 1928. 9:213-215.

[23] Duggar B. M., Karrer J. L. The size of the infective particles in the mosaic disease of tobacco. Ann. Missouri Bot. Gard. 1921. 8:343-356. DOI: 10.2307/2989986 [CrossRef]

[24] Duggar B. M., Armstrong J. K. The effect of treating the virus of tobacco mosaic with the juices of various plants. Ann. Missouri Bot. Gard. 1925. 12:359-366. DOI: 10.2307/2394061 [CrossRef]

[25] Elmer O. H. Inhibition of mosaic infection. Phytopath. 1926. 16:67-68.

[26] Esty R. J. Heat resistance of B. botulinus spores. Abstracts Bact. 1922. 7: 6p.

[27] Falk I. S., Jacobson M. A. Electrophoretic potental, acid and serum agglutination of pneumococci. Jour. Infect. Dis. 1926. 38:182-187. DOI: 10.1093/infdis/38.2.182 [CrossRef]

[28] Falk I. S., Sharp C. G., Link G. K. K. Relation between pH, agglutination and P. D. with Bacterium phaseoli sojense. Proc. Soc. Exp. Biol. and Med. 1926-27. 24: p. 576-578. DOI: 10.3181/00379727-24-3470 [CrossRef]

[29] Goldsworthy M. C. Attempts to cultivate the tobacco mosaic virus. Phytopath. 1926. 17:873-875.

[30] Goldstein Bessie. Cytological studies of living cells of tobacco plants affected with mosaic disease. Torrey Bot. Club Bul. 1924. 51:261-273. DOI: 10.2307/2480309 [CrossRef]

[31] Hastings E. G. A comparison between the resistance to an unfavorable environment of organisms that have grown in native habitat and the same kind grown in artificial culture. Abstracts Bact. 1922. 7:6

[32] Hoggan I. A. Cytological studies on virus diseases of solanaceous plants. Jour. Agr. Res. 1927. 35:651-671.

[33] Holmes F. O. Cytological study of the intracellular body characteristic of Hippeastrum mosaic. Bot. Gaz. 1928. 86:50-57. DOI: 10.1086/333871 [CrossRef]

[34] Iwanowski D. Ueber zwei Krankheiten der Tabakspflanze (Abstr.). Beih. Bot. Centbl. 1893. 3:266-268.

[35] Iwanowski D. Ueber die Mosaikkrankheit der Tabakspflanze. Zeitschr. Pflanzenkrank. 1903. 13:1-44.

[36] Johnson J. The attenuation of plant viruses and the inactivating influence of oxygen. Science. 1926. 64:210 DOI: 10.1126/science.64.1652.210 [CrossRef]

[37] Johnson J. Further studies on the attenuation of plant viruses. Phytopath. 1928. 18:156

[38] Kunkel L. O. Histological and cytological studies on the Fiji disease of sugar cane. Hawaiian Sugar Planters Assoc. Bul. Exp. Sta. Bot. Ser. 1924. 3:99-107.

[39] Kunkel L. O. Studies on aster yellows. Amer. Jour. Bot. 1926. 13:646-705. DOI: 10.2307/2435474 [CrossRef]

[40] Loeb J. On the location of the forces which determine the electrical double layer between collodion particles and water. Jour. Gen. Physiol. 1923-24. 6:105-129. DOI: 10.1085/jgp.6.1.105 [CrossRef]

[41] Loeb J. The influence of the chemical nature of solid particles on their cataphoretic P. D. in aqueous solutions. Jour. Gen. Physiol. 1923-24. 6:215-237. DOI: 10.1085/jgp.6.2.215 [CrossRef]

[42] Mayer A. Ueber die Mosaikkrankheit des Tabaks. Die Landwirtschaftlichen Versuchs-Stationen. 1886. 38:450-467. Abstract in Jour. Mycol. 7: 382-385

[43] McKinley E. B., Fisher R., Holden M. Action of ultra-violet light upon bacteriophage and filterable viruses. Proc. Soc. Exp. Biol. 1925-26. 23:408-412. DOI: 10.3181/00379727-23-2993 [CrossRef]

[44] McKinney H. H. Factors affecting certain properties of a mosaic virus. Jour. Agr. Res. 1927. 35:1-12.

[45] McKinney H. H., Eckerson S. H., Webb R. W. Intracellular bodies associated with a mosaic of Hippeastrum johnsonii. Phytopath. 1923. 13: p. 41-47.

[46] Mulvania M. Cultivation of the virus of tobacco mosaic by the method of Olitsky. Science. 1925. 62:37 DOI: 10.1126/science.62.1593.37 [CrossRef]

[47] Mulvania M. Studies on the nature of the virus of tobacco mosaic. Phytopath. 1926. 16:853-871.

[48] Northrup J. H., de Kruif P. Agglutination of the bacillus of rabbit septicema and of Bacillus typhosus by electrolytes. Jour. Gen. Physiol. 1922. 4:639-667. DOI: 10.1085/jgp.4.6.639 [CrossRef]

[49] Northrup J. H., de Kruif P. The stability of bacterial suspensions. III. Agglutinations in the presence of proteins, normal serum and immune serum. Jour. Gen. Physiol. 1922. 4:655-667. DOI: 10.1085/jgp.4.6.655 [CrossRef]

[50] Olitsky P. K. Experiments on the cultivation of the active agent of tobacco and tomato mosaic. Science. 1924. 60:593-594. DOI: 10.1126/science.60.1565.593 [CrossRef]

[51] Olitsky P. K., Long P. H. Isolation by cataphoresis of virus from vaccinia recovered rabbit. Science. 1929. 49:170 DOI: 10.1126/science.69.1780.170 [CrossRef]

[52] Olitsky P. K., Traum J., Schoening H. W. Report of the Foot and Mouth Commission of the U. S. Dept. Agr. Tech. Bul. 1928. 76:1-172.

[53] Palladin V. I., Livingston B. E. Plant physiology. 1926. Philadelphia: P. Blackiston’s Son and Co. DOI: 10.5962/bhl.title.54922 [CrossRef]

[54] Palm B. T. De Mosaiekziekte van de Tabak een Chlamydozoonose. Deli-Proefstation te Medan. Sumatra Bul. 1922. 15:1-10.

[55] Prausnitz C. Ueber die Natur des d’Herelleschen Phaenomens. Klin. Wochnschr. 1922. 1:1639 DOI: 10.1007/BF01730884 [CrossRef]

[56] Purdy Helen A. Attempts to cultivate an organism from tomato mosaic. Bot. Gaz. 1926. 81:210-217. DOI: 10.1086/333587 [CrossRef]

[57] Purdy Helen A. Multiplication of the virus of tobacco mosaic in detached leaves. Amer. Jour. Bot. 1928. 15:94-99. DOI: 10.2307/2435866 [CrossRef]

[58] Rawlins T. E., Johnson James. Cytological studies of the mosaic disease of tobacco. Amer. Jour. Bot. 1924. 12:19-23. DOI: 10.2307/2435593 [CrossRef]

[59] Sherman H. C., Caldwell M. L., Adams M. Further experiments on the purification of pancreatic amylase. Proc. Soc. Exp. Biol. and Med. 1926. 23:413-416. DOI: 10.3181/00379727-23-2994 [CrossRef]

[60] Severin H. H. P. Minimum incubation period of causative agent of curly-leaf in beet leaf hopper and sugar beets. Phytopath. 1921. 11:424-429.

[61] Smith F. F. Some cytological and physiological studies of mosaic diseases and leaf variations. Ann. Missouri Bot. Gard. 1927. 13:425-484. DOI: 10.2307/2394029 [CrossRef]

[62] Smith J. H. Experiments with a mosaic disease of tomato. Ann. Applied Biol. 1928. 15:155-167. DOI: 10.1111/j.1744-7348.1928.tb07044.x [CrossRef]

[63] Smith R. E., Boncquet P. A. Connection of a bacterial organism with curly-leaf of sugar beet. Phytopath. 1915. 5:335-342.

[64] Todd C. On the electrical behavior of the bacteriophage. Brit. Jour. Exp. Path. 1927. 8:369-376.

[65] Vinson C. G., Petre A. W. Mosaic disease of tobacco: purification. Bot. Gaz. 1929. 87:14-37. DOI: 10.1086/333922 [CrossRef]

[66] Waksman S. A., Davison W. C. Enzymes. 1926. Baltimore: Williams and Wilkins Co. DOI: 10.1097/00010694-192701000-00008 [CrossRef]

[67] Walker M. N. A comparative study of the mosaic diseases of cucumber, tomato and Physalis. Phytopath. 1926. 16:431-458.

[68] Willstatter R., Graser J., Kuhn R. Zur Kenntnis des Invertins. Hoppe Seylers Zeitschrift fuer Physiologische Chemie. 1922. 123:1-78. DOI: 10.1515/bchm2.1922.123.1-3.1 [CrossRef]

[69] Winslow C. E. A., Falk I. S., Caulfield M. F. Electrophoresis of bacteria as influence by hydrogen ion concentration and the presence of sodium and calcium salts. Jour. Gen. Physiol. 1924. 6:177-200. DOI: 10.1085/jgp.6.2.177 [CrossRef]

[70] Winslow C. E. A., Shaughnessy H. J. The alkaline isopotential point of bacterial cells. Jour. Gen. Physiol. 1924. 6:697-701. DOI: 10.1085/jgp.6.6.697 [CrossRef]

Takahashi W, Rawlins T. 1930. Electrophoresis of tobacco mosaic virus. Hilgardia 4(15):441-463. DOI:10.3733/hilg.v04n15p441
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