University of California

Production of the perfect stage of Mycena citricolor (Berk. and Curt.) Sacc.


J. A. Salas
J. G. Hancock

Authors Affiliations

J. A. Salas was former Research Assistant in Plant Pathology, Berkeley, is a Plant Pathologist employed by the United Fruit Company, La Lima, Honduras; J. G. Hancock was Associate Professor of Plant Pathology and Associate Plant Pathologist, Agricultural Experiment Station, Berkeley.

Publication Information

Hilgardia 41(9):213-234. DOI:10.3733/hilg.v41n09p213. February 1972.

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The taxonomy and biology of Mycena citricolor (Berk and Curt.) Sacc. is reviewed and techniques of induction of basidiocarps by certain other fungi are described.

Mycena citricolor was induced to produce the perfect stage when grown in co-culture with Penicillium oxalicum, P. palitans, P. cyclopium, P. brevi-compactum, or P. viridicatum. Basidiocarp yield was highest when M. citricolor was grown in co-culture with P. oxalicum. A low percentage of field isolates of M. citricolor were able to produce some basidiocarps in mono-culture, but yield was greatly increased when this fungus was grown in co-culture with P. oxalicum. Other isolates of M. citricolor produced basidiocarps only when grown in co-culture with P. oxalicum. However, several other basidiomycetes failed to produce the perfect stage when grow in co-culture with P. oxalicum.

A basidiocarp-stimulating substance(s) (BSS) was present in sterile supernatant fluids from cultures of Penicillium oxalicum. BSS resisted autoclaving and readily passed through dialysis tubing. Yield of BSS was highest when P. oxalicum was cultured in potato dextrose broth (PDB) or Emerson’s YpSs medium in still culture for 1 week under laboratory conditions. P. oxalicum grown in PDB produced BSS independently of light conditions, but M. citricolor produced basidiocarps in response to BSS (or in co-culture with P. oxalicum) only when grown in alternating periods of light and dark. M. citricolor produced basidiocarps only when it was cultured on natural or semi-synthetic media.

Literature Cited

Ashby S. F. The perfect form of Stilbum flavidum Cke. in pure culture. Roy. Bot. Gardens Kew Bul. Misc. Inf. No. 1925. 8:325-28.

Barquero M. Control of Omphalia leaf spot of coffee in Costa Rica puratized agricultural spray. Plant Dis. Reptr. 1955. 39:582

Bianchini C. L., Soto C. A., Rodriguez R. A. Uso de fungicidas a base de arsénico en el café. (Resumen de una conferencia dictada el 5 de mayo de 1958 en San José.) San José. 1958. Costa Rica: Ministerio de Agricultura c Industrias. 5p. (Mimeografiado.)

Buller A. H. R. Researches on fungi. 1934. 6: New York: Longmans Green and Co. p. 397-443.

Carvajal B. F. Ojo de gallo (Omphalia flavida). Rev. Inst. Defensa Café (Costa Rica). 1939. 7:535-76.

Castaño A. J. J. Principales causas predisponentes para la enfermedad de la gotera en nuestros cafetales. Rev. Cafetera Colombia. 1951. 10:3750-56.

Castaño A. J. J. El arseniato de plumo (Du Pont Nu Rexform) en el control de la gotera del cafeto. Rev. Cafetera Colombia. 1957. 13:36-44.

Cochrane V. W. Physiology of Fungi. 1958. New York: John Wiley and Sons, Inc. 524p.

Dennis R. W. G. An earlier name for Omphalia flavida Maubl. and Rang. Kew Bul. 1950. 3:434 DOI: 10.2307/4109441 [CrossRef]

Diaz Moreno J. Comportamiento de varios fungicidas en el combate de Mycena citricolor en Ecuador. Turialba. 1961. 11:93-97.

Echandi E. Inhibition of gemmae (cabecitas) production of Mycena citricolor on coffee trees. Plant Dis. Reptr. 1956. 40:775

Echandi R., Echandi E. Influencia de vitaminas y amino ácids en el crecimiento y esporulación de Mycene citricolor, Phoma costarricensis, Cercospora coffeicola y Ceratostomella fimbriata. Rev. Biol. Trop. (Univ. Costa Rica). 1958. 6:103-11.

Echandi E., Segall R. H. The effectiveness of certain eradicant fungicides on inhibition of gemmae of Mycena citricolor. Phytopathology. 1958. 48:11-14.

Fawcett G. L. Fungus diseases of coffee in Porto Rico. Porto Rico Agri. Exp. Sta. Bul. 1915. 17:1-29.

Foster J. W. The heavy metal nutrition of fungi. Bot. Rev. 1939. 5:207-39. DOI: 10.1007/BF02878490 [CrossRef]

Fowlks E. R., Leben C., Snell J. F. Sterols in relation to the influence of nystatin on Pythium aphanidermatum and Colletotrichum lagenarium. Phytopathology. 1967. 57:246-49.

Fries N. Growth factor requirements of some higher fungi. Svensk Bot. Tidskriff. 1950. 44:379-86.

Grant C. L., Pramer D. Minor elements composition of yeast extract. Jour. Bacteriol. 1962. 84:869-70.

Hawker L. E. Fungi and vitamins. Roy. Coll. Sci. Jour. 1944. 14:65-78.

Hawker L. W., Ainsworth G. C., Sussman A. S. Environmental influences on reproduction. The Fungi, An Advanced Treatise. 1966. New York: Academic Press. p. 435-69. 805 pp

Hayes W. A., Randle Phyllis E., Last F. T. The nature of microbial stimulus affecting sporophore formation in Agaricus bisporus (Lange) Sing. Ann. Appl. Biol. 1969. 64:177-87. DOI: 10.1111/j.1744-7348.1969.tb02867.x [CrossRef]

Klebs G. Zur physiologie der Fortflanzung einiger Pilze. Z. Wiss. Bot. 1898. 32:1-70.

Klebs G. Zur physiologie der Fortflanzung einiger Pilze. Z. Wiss. Bot. 1899. 33:513-97.

Klebs G. Zur physiologie der Fortflanzung einiger Pilze. Z. Wiss. Bot. 1900. 35:80-203.

Leonard T. J., Dick S. Chemical induction of haploid fruiting bodies in Schizophyllum commune. Proc. Nat. Acad. Sci. 1968. 59:745-51. DOI: 10.1073/pnas.59.3.745 [CrossRef]

Maublanc A., Rangel E. Le Stilbum flavidum Cooke, forme avorteé de l’ Omphalia flavida n. sp. Bull. Soc. Mycol. (France). 1914. 30:41-47.

Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum. 1962. 15:473-97. DOI: 10.1111/j.1399-3054.1962.tb08052.x [CrossRef]

Park J. T., Agnihotri V. P. Bacterial metabolites trigger sporophore formation in Agaricus bisporus. Nature. 1969. 222:94 DOI: 10.1038/222984a0 [CrossRef]

Perez V. M. Control del ojo de gallo por medio de fungicidas. Suelo Tico (Costa Rica). 1952. 6:264-71.

Phillips D. J. Carnation shoot tip culture. Colorado Agr. Exp. Sta. Tech. Bull. 1968. 102:1-22.

Porter C. L., Carter J. C. Competition among fungi. Bot. Rev. 1938. 4:165-82. DOI: 10.1007/BF02870112 [CrossRef]

Puttemans M. A. Sur la maladie du cafeier produite par le Stilbella flavida. Bull. Soc. Mycol. (France). 1904. 20:157-65.

Quirós M. Ojo de gallo (Omphalia flavida Maubl &; Range.). Suelo Tico (Costa Rica). 1951. 5:65-69.

Raper J. R. Chemical regulation of sexual processes in the thallophytes. Bot. Rev. 1952. 18:447-545. DOI: 10.1007/BF02957551 [CrossRef]

Raper J. R. The control of sex in fungi. Amer. Jour. Bot. 1960. 47:794-808. DOI: 10.2307/2439117 [CrossRef]

Raper J. R., Krongelb G. S. Genetic and environmental aspects of fruiting in Schizophyllum commune Fr. Mycologia. 1958. 50:707-40. DOI: 10.2307/3756180 [CrossRef]

Robbins W. J. A survey of the growth requirements of some basidiomycetes. Mycologia. 1950. 42:470-76. DOI: 10.2307/3755562 [CrossRef]

Robbins W. J., Kavanagh Virgene. Vitamin deficiencies of the filamentous fungi. Bot. Rev. 1942. 8:411-71. DOI: 10.1007/BF02881123 [CrossRef]

Rodriguez C. J. Jr. Physiological studies on the American leaf spot of coffee and on its causal agent Mycena citricolor. I. Metabolic studies on the leaf spot and on M. citricolor. II. Effect of light on gemmae formation in M. citricolor 1964. p.71. Ph.D. Thesis, Univ. Wisconsin, Madison

Rodriguez C. J. Jr., Arny D. C. Effect of light on gemmae formation in Mycena citricolor. Can. Jour. Bot. 1967. 45:675-79. DOI: 10.1139/b67-074 [CrossRef]

Salas J. A. Experimentos comparativos entre el arseniato de plomo y algunos fungicidas y antibióticos en el combate del ojo de gallo, Mycena citricolor (Berk. and Curt.) Sacc. en café (Coffea arabica L.) 1960. p.45. M. A. Thesis, Turrialba, Costa Rica, Instituto Interamericano de Ciencias Agricolas

Salas J. A. Studies on the production of the perfect stage of Mycena citricolor (Berk. and Curt.) Sacc 1970. p.117. Ph.D. Thesis, Univ. California, Berkeley

Sequeira L. Studies on Omphalia flavida, the agent of the American leaf spot disease of coffee 1952. p.288. Ph.D. Thesis, Harvard Univ.

Sequeira L. Nuclear phenomena in the basidia and basidiospores of Omphalia flavida. Mycologia. 1954. 46:470-83.

Sequeira L. The host range of Mycena citricolor (Berk &; Curt.). Sacc. Turrialba. 1958. 8:136-47.

Sequeira L., Steeves A. Auxin inactivation and its relation to leaf drop caused by the fungus Omphalia flavida. Plant Physiol. 1954. 29:11-16. DOI: 10.1104/pp.29.1.11 [CrossRef]

Singer R. The agaricales (mushrooms) in modern taxonomy. Lilloa. 1949. 22:1-832.

Singer R. Mushrooms and truffles. Botany Cultivation and Utilization. 1961. New York: Interscience Publishers, Inc. 272p.

Steinberg R. A. Growth of fungi in synthetic nutrient solutions. Bot. Rev. 1939. 5:327-50. DOI: 10.1007/BF02878505 [CrossRef]

Steinberg R. A. Growth of fungi in synthetic nutrient solutions. II. Bot Rev. 1950. 16:208-28.

Taber W. A., Ainsworth G. C., Sussman A. S. Morphogenesis in basidiomycetes. The Fungi. 1966. 2: New York: Academic Press. p. 387-412.

Trione E. J., Leach C. M., Mutch J. T. Sporogenic substances isolated from fungi. Nature. 1966. 212:163-64. DOI: 10.1038/212163a0 [CrossRef]

Urayama T. Preliminary note on the stimulative effect of certain specific bacteria upon fruit body formation in Psilocybe panaeoliformis Murrill. Bot. Mag. (Tokyo). 1957. 70:29-30.

Urayama T. Studies on fruit body formation of Psilocybe panaeoliformis Murrill in pure culture. Mem. Fac. Liberal Arts Educ. Miyazaki Univ. 1960. 9:393-462.

Urayama T. Initiation of pinheads in Psilocybe panaeoliformis caused by certain bacteria. Mushroom Sci. 1965. 4:141-56.

Uribe Arango H. La gotera del café Rev. Fac. Nac. Agron. (Colombia). 1947. 7:249-60.

Volz D. A., Beneke E. J. Nutritional regulation of basidiocarp formation and mycelial growth of Agaricales Mycopathol. Mycol. Appl. 1969. 37:225-53. DOI: 10.1007/BF02051357 [CrossRef]

Wellman F. L. Dissemination of Omphalia leaf spot of coffee. Turrialba. 1950. 1:12-27.

Wellman F. L. Coffee. Botany cultivation and utilization. 1961. New York: Interscience Publishers, Inc. 488p.

Salas J, Hancock J. 1972. Production of the perfect stage of Mycena citricolor (Berk. and Curt.) Sacc.. Hilgardia 41(9):213-234. DOI:10.3733/hilg.v41n09p213
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