Studies on Diplodia and Diplodia-like fungi: IV. Effects of pH, temperature, light, and vitamins on certain taxonomic
      characters

Hewitt, W; Webster, R; Satour, M

Studies on Diplodia and Diplodia-like fungi: IV. Effects of pH, temperature, light, and vitamins on certain taxonomic characters

Authors

W. B. Hewitt
R. K. Webster
M. M. Satour

Authors Affiliations

W. B. Hewitt was Professor of Plant Pathology and Plant Pathologist in the Experiment Station, Davis, and Assistant Director, Agricultural Field Stations, San Joaquin Valley Research and Extension Center, Parlier; R. K. Webster was Associate Professor of Plant Pathology and Associate Plant Pathologist in the Experiment Station, Davis; M. M. Satour was Postgraduate Research Plant Pathologist, Department of Plant Pathology, Davis. He is now with the Department of Plant Pathology, Ministry of Agriculture, Orman, Giza, United Arab Republic.

Publication Information

Hilgardia 41(5):77-94. DOI:10.3733/hilg.v41n05p077. November 1971.

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Abstract

Fifteen isolates of Diplodia and Diplodia-like fungi were grown on various synthetic media for study of the effects of pH, temperature, light, and vitamins on growth, sporulation, and stability of morphological characters currently used in identification. The pH of the culture medium within ranges for good growth had little effect on stabilizing the characteristics used in classification. Light, either continuous or cyclic, favored growth and maturity more than did continuous dark, although some isolates did grow under the latter condition. Two isolates required biotin for sporulation. All others tested grew and sporulated on minimal medium without biotin or other added vitamins. Potato-dextrose-agar is considered a satisfactory medium for growing these fungi for identification purposes.

Literature Cited

Cochrane Vincent W. Physiology of fungi. 1958. New York: John Wiley &; Sons, Inc. 524p.

Lilly V. G., Barnett H. L. Physiology of the fungi. 1951. New York: McGraw Hill Book Co. 464p.

Satour M. M., Webster R. K., Hewitt W. B. Studies on Diplodia and Diplodia-like fungi. I. Effects of carbon sources on certain taxonomic characters and on growth in agar culture. Hilgardia. 1969a. 39(22):601-29. DOI: 10.3733/hilg.v39n22p601 [CrossRef]

Satour M. M., Webster R. K., Hewitt W. B. Studies on Diplodia and Diplodia-like fungi. II. Effects of nitrogen sources on growth, sporulation, and certain taxonomic characters. Hilgardia. 1969b. 39(22):631-53. DOI: 10.3733/hilg.v39n22p631 [CrossRef]

Webster R. K., Hewitt W. B., Polach F. J. Studies on Diplodia and Diplodia-like fungi. III. Variation in Diplodia natalensis from grape in California. Hilgardia. 1969. 39(22):655-71. DOI: 10.3733/hilg.v39n22p655 [CrossRef]

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Studies on Diplodia and Diplodia-like fungi: VI. Effects of natural substrates on variability in taxonomic characters