A technique for embryo culture of peaches

Gilmore, A

A technique for embryo culture of peaches

Author

A. E. Gilmore

Author Affiliations

A. E. Gilmore was Assistant Specialist in the Experiment Station.

Publication Information

Hilgardia 20(8):147-170. DOI:10.3733/hilg.v20n08p147. September 1950.

PDF of full article, Cite this article

Abstract

This publication describes a technique. …

for growing peach-embryo cultures. In laboratory experiments, a high percentage of the cultures was free of microorganisms that would develop under yeast-extract broth within 15 days at room temperature.

The salient feature of the technique is a “sterilization” procedure on dry, dormant peach seeds, followed by maintenance of aseptic conditions during the subsequent after-ripening. Merthiolate 1:2,000 in 50 per cent alcohol was found to be suitable for the purpose.

Various factors influencing the growth of peach-embryo cultures are discussed. Suggestions are also made for establishing a standard practice in growing peach-embryo cultures to be used for biological testing.

Literature Cited

Flemion Florence. Dwarf seedlings from non-after-ripened embryos of peach, apple and hawthorn. Boyce Thompson Inst. for Plant Research, Contrib. 1934. 6:205-9.

Haut I. C., Gardner F. E. The influence of pulp disintegration upon the viability of peach seeds. Amer. Soc. Hort. Sci. Proc. 1934. 32:323-27.

Hoagland D. R., Arnon D. I. The water-culture method for growing plants without soil. California Agr. Exp. Sta. Cir. 1938. 347:1-37. https://archive.org/details/waterculturemeth347hoag

LaRue Carl D. The use of bromine in the sterilization of fruits and seeds. Science. 1937. 85:319

Proebsting E. L., Gilmore A. E. The relation of peach root toxicity to the re-establishing of peach orchards. Amer. Soc. Hort. Sci. Proc. 1941. 38:21-26.

Scott H. D., Waugh J. G., Cullinan F. P. An injurious effect of peach juice on germination of the seed. Amer. Soc. Hort. Sci. Proc. 1942. 40:283-85.

Tukey H. B. Artificial culture of sweet cherry embryos. Jour. Heredity. 1933. 24:7-12.

Artificial culture methods for isolated embryos of deciduous fruits. Amer. Soc. Hort. Sci. Proc. 1934. 32:313-22.

The excised-embryo method of testing the germinability of fruit tree seed with particular reference to peach seed. Amer. Soc. Hort. Sci. Proc. 1944. 45:211-19.

White Philip R. A handbook of plant tissue culture. 1943. Lancaster, Pennsylvania: The Jacques Cattell Press. 277p. DOI: 10.1097/00010694-194308000-00010 [CrossRef]

Wilson James K. Calcium hypochlorite as a seed sterilizer. Amer. Jour. Bot. 1915. 2:420-27. DOI: 10.2307/2435014 [CrossRef]

Also in this issue:

Productivity improvement with: Picker aids for grape harvesting

Nitrogen: In relation to tomato quality

New evidence: Light dependency in seed germination

Control of hornflies on range cattle with systemic insecticides

Organic acid synthesis in lemon fruits

Control of citrus blast in northern California

Nitrogen utilization: By growing lambs fed normal, low protein, or nitrogen-enriched cottonseed meal

High temperature effects: On sugar beet germination in Imperial Valley