The effects of zinc and iron salts on the cell structure of mottled orange leaves
AuthorsH. S. Reed
Authors AffiliationsH. S. Reed was Professor of Plant Physiology in the Citrus Experiment Station and Graduate School of Tropical Agriculture and Plant Physiologist in the Experiment Station; J. Dufrénoy was International Fellow, Rockefeller Foundation.
Hilgardia 9(2):111-141. DOI:10.3733/hilg.v09n02p111. January 1935.
Abstract does not appear. First page follows.
The term “mottle-leaf“ designates a functional disease of certain species and varieties of the genus Citrus. The most evident symptom of this disease is the absence of chlorophyll from certain areas between the veins of the leaf and, in more advanced stages, dwarfing of the leaves. The exact cause, or causes, of mottle-leaf have not been determined, but the evidence at hand supports the belief that the disease is independent of parasitic microörganisms. Climatic conditions often influence the occurrence and intensity of the disease, but it cannot be said that they have been shown to be the determining factor.
Soil conditions have been found to be more directly related to the occurrence of mottle-leaf. The application of nitrates (Vaile(20) or of excessive amounts of urea (Haas(7)) may produce mottling of orange-tree foliage. The ratio of calcium to potassium also has certain pertinent relations to the disease (Kelley and Cummins,(10) Reed and Haas(17)), yet applications of calcium to the soil are by no means a corrective. Frequent applications of organic manures have been found to be one of the most satisfactory means in California for holding in check this disease, or for ameliorating the condition of trees badly affected with mottle-leaf.
The use of iron and zinc salts for the control of functional diseases of fruit trees has received much attention in California in recent years. Peach trees affected with little-leaf (Chandler, Hoagland, and Hibbard(5)) and orange trees affected with mottle-leaf (Johnston(9)) have shown striking benefits from applications of zinc sulfate singly or in combination with iron sulfate.
Mazé(12) pointed out a significant relation between zinc and sulfur metabolism in maize. He found that roots which were grown in a solution deficient in zinc were soon coated with an ocherous deposit.
 Bertrand G., Andreitcheva M. Sur la teneur comparée en zinc des feuilles vertes et des feuilles étiolées. Compt. Rend. Acad. Sci. [Paris]. 1933. 197:1374-1376.
 Bertrand G., Benzon B. Sur la teneur en zinc des principaux aliments d’origine végétale. Ann. Inst. Pasteur. 1929. 43:386-393.
 Bradley H. C. A delicate color reaction for copper and a microchemical test for zinc. Amer. Jour. Sci. 1906. 22:326
 Chamot E. M., Mason C. W. Handbook of chemical microscopy. 1931. 2:xxii + 885 pages. Illus. J. Wiley and Sons, New York
 Chandler W. H., Hoagland D. R., Hibbard P. L. Little-leaf or rosette of fruit trees. II. Effect of zinc and other treatments. Amer. Soc. Hort. Sci. Proc. 1932. 29:255-263.
 Giroud A., Bulliard H. Réaction des substances à function sulfhydryle. Méthode de mise en evidence dans les tissus. Protoplasma. 1933. 19:381-384.
 Haas A. R. C. Some nutritional aspects in mottle-leaf and other physiological diseases of citrus. Hilgardia. 1932. 6(15):483-559. DOI: 10.3733/hilg.v06n15p483 [CrossRef]
 Hammond Wesley H. A rapid method for the detection of zinc in the presence of iron. Chem.-Anal. 1928. 17:14
 Johnston J. C. Zinc sulfate, promising new treatment for mottle-leaf. California Citrograph. 1933. 18:107 116-118
 Kelley W. P., Cummins A. B. Composition of normal and mottled citrus leaves. Jour. Agr. Research. 1920. 20:161-191.
 Mangin L. Recherches sur la distribution des composés pectiques chez les végétaux. Jour. Botanique 5:400; 6:206, 238. 1891-1893. 7:336-337.
 Mazé P. Recherches de physiologie végétale. IV. Influences respectives des éléments de la solution minérale sur le développement du mais. Ann. Inst. Pasteur. 1914. 28:21-68.
 Penzig O. Studi botanici sugli agrumi e sulle piante affini 1887. p.590. p. and atlas of 58 pl. Tipographia eredi Botta, Roma
 Policard A. Sur un méthode de microincineration applicable aux recherches histochimiques. Bul. Soc. Chim. France 4eme ser. 1923. 33:1551
 Reed H. S., Dufrénoy J. Effets de l’affection dite “mottle-leaf” sur la structure cellulaire des Citrus. Revue Gén. Bot. 1935. 46:33-44.
 Reed H. S., Dufrénoy J. Modifications in cell structure accompanying mottle-leaf of the orange. Amer. Jour. Bot. 1934. (In press.)
 Reed H. S., Haas A. R. C. Nutrient and toxic effects of certain ions on citrus and walnut trees, with especial reference to the concentration and pH of the medium. California Agr. Exp. Sta. Tech. Paper. 1924. 17:1-75.
 Rufz de Lavison J. Recherches sur la pénétration des sels dans le protoplasme et sur la nature de leur action toxique. Ann. Sci. Nat., Bot. 1911. 14:97-193.
 Tupper-Carey R. M., Priestley J. H. The composition of the cell-wall at the apical meristem of stem and root. Roy. Soc. [London] Proc. Series B. 1923. 95:109-131.
 Vaile R. S. Fertilizer experiments with citrus trees. California Agr. Exp. Sta. Bul. 1922. 345:465-512. http://archive.org/details/fertilizerexperi345vail
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